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Tritium gas exchange

Tritium gas exchange using a hydrogen transfer catalyst can be employed in labelling compounds such as carbohydrates, nucleosides, nucleotides, alkaloids, amino acids and compounds containing benzylic protons. This technique achieves specific activities of 1-50 Ci/mmol. Tritium gas exchange using a hydrogen transfer catalyst includes the following:

- The tritiation of your desired target molecule with carrier-free tritium gas exposure of your compound to the appropriate amount of carrier-free tritium gas and catalyst.

- Complete removal of labile tritium.

- Assay of the crude product for total activity.

Exchange with tritiated water

Tritiated water in acidic, basic or neutral solution can be used to label compounds such as nucleotides, pyrimidines and alkaloids. This technique usually achieves specific activities of 0.5-30 Ci/mmol. Exchange with tritiated water includes the following:

- Reaction of your compound with the appropriate amount of tritiated solvent.

- Complete removal of labile tritium.

- Assay of the crude product for total activity.

Exposure to tritium gas (Wilzbach method)

Labelling by exposure to tritium gas, known as the Wilzbach method, can be performed on some pharmaceutical and other complex compounds with satisfactory results, however many compounds will suffer significant radiation induced decomposition and achieve low specific activities. This method should be used only if the compound cannot be labelled using other less destructive methods. Gas exposure tritium labelling includes the following:

- Exposure of your compound to 15 Ci of carrier-free tritium gas.

- Complete removal of labile tritium.

- Assay of the crude product for total activity.